USE OF CAPILLARY ZONE ELECTROPHORESIS FOR FISH SPECIES IDENTIFICATION- DIFFERENTIATION OF FLATFISH SPECIES

A capillary zone electrophoresis (CZE) method is described for the ana lysis of fish sarcoplasmic proteins at low pH. Aqueous extracts of eig ht flatfish species, containing the sarcoplasmic proteins, were subjec ted to the action of an electric field in a capillary electrophoresis apparatus; the separated proteins were detected by means of an UV dete ctor after less than 35 min. Acceptable resolution and reproducibility were obtained using low protein concentration (0.1 mg/mL) in the extr acts analyzed. The CZE protein profiles that resulted were specific fo r each species and could be employed for differentiation and identific ation purposes.