CHROMATOGRAPHIC ISOLATION OF ANTIOXIDANTS GUIDED BY A METHYL LINOLEATE ASSAY

Authors
BERTELSEN G CHRISTOPHERSEN C NIELSEN PH MADSEN HL STADEL P
Citation
G. Bertelsen et al., CHROMATOGRAPHIC ISOLATION OF ANTIOXIDANTS GUIDED BY A METHYL LINOLEATE ASSAY, Journal of agricultural and food chemistry, 43(5), 1995, pp. 1272-1275
Citations number
34
Categorie Soggetti
Food Science & Tenology",Agriculture,"Chemistry Applied
Journal title
Journal of agricultural and food chemistry → ACNP
ISSN journal
00218561
Volume
43
Issue
5
Year of publication
1995
Pages
1272 - 1275
Database
ISI
SICI code
0021-8561(1995)43:5<1272:CIOAGB>2.0.ZU;2-E
Abstract
A convenient and fast method for establishing the relative antioxidant activity of chromatographically purified fractions of plant extracts has been developed. The assay electrochemically determines the myoglob in-catalyzed oxygen consumption following addition of the fractions to methyl linoleate. Since the oxygen consumption rate decreases with in creasing antioxidant activity, the method can be used for systematic s creening of naturally occurring antioxidants. Applying this method to extracts of Satureja hortensis led to isolation of rosmarinic acid.