EXPERIMENTAL FOREIGN-BODY INFECTION IN THE BILIARY-TRACT IN RATS

Citation
Jl. Yu et al., EXPERIMENTAL FOREIGN-BODY INFECTION IN THE BILIARY-TRACT IN RATS, Scandinavian journal of gastroenterology, 30(5), 1995, pp. 478-483
Citations number
36
Categorie Soggetti
Gastroenterology & Hepatology
ISSN journal
00365521
Volume
30
Issue
5
Year of publication
1995
Pages
478 - 483
Database
ISI
SICI code
0036-5521(1995)30:5<478:EFIITB>2.0.ZU;2-L
Abstract
Background: Biomaterials used for biliary drainage may potentially res ult in biomaterial-associated infections. Methods: Foreign-body infect ion in the biliary tract was investigated in rats. Rubber drain pieces were implanted in the biliary tract in rats for 1-4 weeks, followed b y challenges with 10(2) to 10(4) colony-forming units (cfu) Escherichi a coli injected into the common bile duct. The rate of infection was c alculated, the bacterial growth in the biliary tract was observed over 72 h after challenges, and the opsonic activity in bile and in sera w as assessed. Results: In the group with drain material, inocula as sma ll as 10(2) cfu produced persisting infection in the common bile duct in 90% of animals, whereas the same number of E. coli infected only 30 % of rats in the control and sham implantation groups (p < 0.01, chi-s quare test). Complement-mediated opsonic activity in bile of animals w ith implanted drain pieces gradually decreased with time, whereas opso nic activity in sera from the same animals remained unchanged. Conclus ions: Implants in the biliary tract impair local host defense, resulti ng in an increased susceptibility to microbial infection.