Various approaches have been developed in recent years at the Soil Bio
logy Department of Moscow State University for assessing microbial div
ersity in soil ecosystems. The geographical approach involves comparat
ive taxonomic analysis of microbial populations in different natural z
ones. The stratigraphic approach involves simultaneous identification
of microbial communities throughout all biogeocenotic layers, that is,
phyllosphere, aboveground substrates (mosses, litter, etc.), and soil
horizons. The microlocal approach involves determination of spatial r
estrictions for specific microbial groups. The successional approach i
nvolves temporal monitoring of microbial community structure. These ap
proaches elucidate the spatial and temporal specificity of microbial c
ommunities, substantially extend the diversity of studied microbial gr
oups and ecological niches, and provide a theoretical basis for direct
ed screening of microbial taxa in natural substrates.