CHIMERIC FLAVONOL SULFOTRANSFERASES DEFINE A DOMAIN RESPONSIBLE FOR SUBSTRATE AND POSITION SPECIFICITIES

The pFST3 and pFST4' cDNAs encode flavonol sulfotransferases (ST) that are 69% identical in amino acid sequence yet exhibit strict substrate and position specificities. To determine the domain responsible for t he properties of the flavonol STs, several chimeric flavonol STs were constructed by the reciprocal exchange of DNA fragments derived from t he plasmids pFST3 and pFST4' and by the expression of the correspondin g chimeric proteins in Escherichia coli. The chimeric enzymes were enz ymatically active even though their activities were reduced compared t o the parent enzymes. The specificity of the resulting hybrid proteins indicates that an interval of the flavonol STs spanning amino acids 9 2-194 of the flavonol 3-ST sequence contains the determinant of the su bstrate and position preferences. From the comparison of the amino aci d sequences between plant and animal STs, this interval can be subdivi ded into a highly conserved region corresponding to positions 134-152 of the flavonol 3-ST, flanked by two regions of high divergence from 9 8 to 110 and 153 to 170. In view of the similarities in length and hyd ropathic profiles as well as the presence of four conserved regions be tween plant and animal STs, the results of these experiments suggest t hat this interval is involved in the recognition of substrates and/or catalysis in all STs.