O. Vincent et Jm. Gancedo, ANALYSIS OF POSITIVE ELEMENTS SENSITIVE TO GLUCOSE IN THE PROMOTER OFTHE FBP1 GENE FROM YEAST, The Journal of biological chemistry, 270(21), 1995, pp. 12832-12838
We have identified in the promoter of the FBP1 gene from Saccharomyces
cerevisiae, which codes for fructose-1,6-bisphosphatase, two elements
which can form specific DNA . protein complexes and which confer gluc
ose-repressed expression to an heterologous reporter gene. Complex for
mation and activation of transcription by either element require a fun
ctional CAT1 gene and are not blocked by a hap2-1 mutation, although t
his mutation interferes with maximal expression of the FBP1 gene. A se
quence from one of the elements acts as a weak upstream activating seq
uence, but its activity can be stimulated up to 10-fold by neighboring
sequences. A further element of the promoter has been characterized,
which forms a specific DNA . protein complex only when a nuclear extra
ct from derepressed cells is used. This element does not activate tran
scription in a heterologous promoter. The DNA sequences of the three e
lements involved in protein binding, defined by DNase I footprinting,
have no homology with consensus sequences for known activating factors
.