B. Keita et al., RATE-LIMITING ONE-ELECTRON TRANSFER IN THE OXIDATION OF NADH BY POLYOXOMETALATES, Chemical physics letters, 237(5-6), 1995, pp. 411-418
The kinetics of NADH oxidation by 3 Dawson-type mixed heteropolyanions
were studied in buffered aqueous pH = 7 medium, by the stopped flow t
echnique and UV-visible spectroscopy. The log of k was a linear functi
on of the E(0) of the first redox systems of the heteropolyanions with
a slope of 16.5 V-1. The results indicate that, in the present case,
the oxidation of NADH proceeds by a multistep mechanism involving init
ial rate-limiting one-electron transfer. An estimate of the E(0) value
for the one-electron NADH/NADH(+.) couple has been obtained.