Jem. Beurskens et al., DEHALOGENATION OF CHLORINATED DIOXINS BY AN ANAEROBIC MICROBIAL CONSORTIUM FROM SEDIMENT, Environmental toxicology and chemistry, 14(6), 1995, pp. 939-943
Anaerobic microorganisms enriched from Rhine River sediments are able
to remove chlorine substituents from polychlorinated dibenzo-p-dioxine
s (PCDDs). A model PCDD, 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-
TeCDD) was reductively dechlorinated to both 1,2,3- and 1,2,4-trichlor
odibenzo-p-dioxins (1,2,3- and 1,2,4-TrCDD). These compounds were furt
her dechlorinated to 1,3- and 2,3-dichlorodibenzo-p-dioxins and traces
of 2-monochlorodibenzo-p-dioxin. This is the first report in the lite
rature of the anaerobic microbial dechlorination of PCDDs. The same en
richment culture was previously found to dechlorinate chlorinated benz
enes (CBs) and polychlorinated biphenyls (PCBs). An anaerobic culture
able to remove aryl chlorines from three classes of compounds has not
been reported before. The rate at which the culture dechlorinates 1,2,
3,4-TeCDD (t(1/2)=15.5 d) was between those observed for CBs and PCBs.
This study shows that reductive dechlorination may have an effect on
PCDDs in sediments, as has been demonstrated for CBs and PCBs. The for
mation of metabolites with a conserved 2,3-substitution pattern from 1
,2,3,4-TeCDD indicates that dechlorination of highly chlorinated diben
zo-p-dioxins may result in metabolites that are potentially more toxic
than the parent compounds.