PARTITION-COEFFICIENTS OF CATECHOL AND CA TECHOLAMINES IN AQUEOUS 2-PHASE SYSTEM CONTAINING POLYETHYLENE-GLYCOL AND AMMONIUM-SULFATE

The partition coefficients of catechol and three kinds of catecholamin es were determined in aqueous two-phase systems containing polyethylen e glycol (M(w)=7,500) ammonium sulfate at 25 degrees C. It was found t hat the distribution coefficients of catechol and catecholamines incre ase with increasing tie-line length and that an approximately linear r elationship exists between lnK(4) (K-4=m(4)(TOP)/m(4)(BOT)) and tie-li ne length. The partition coefficients of catecholamine were rather low er than these of catechol. Further, the osmotic virial equation was us ed to correlate the partition coefficients of catechol and three kinds of catecholamines.