CONTRIBUTING FACTORS TO POOR FUNCTIONAL RECOVERY AFTER DELAYED NERVE REPAIR - PROLONGED AXOTOMY

The contribution of prolonged motoneuron axotomy to the poor functiona l recovery after delayed nerve repair was determined by means of a ner ve cross-anastomosis paradigm in the rat, The tibial nerve was axotomi zed up to 12 months before it was cross-sutured to the distal stump of the freshly cut common peroneal nerve to innervate the freshly denerv ated tibialis anterior muscle, Three to 17 months later, muscle and mo tor unit (MU) forces were measured to quantify the number of axons tha t had successfully regenerated and reinnervated the muscle, The extent of axonal branching was estimated by the innervation ratio (IR) (i.e. , the number of muscle fibers innervated by each axon), which was obta ined directly by counting muscle fibers in a single glycogen-depleted MU in each muscle and indirectly by calculation, The total number of M Us in each muscle significantly decreased with progression of axotomy and was only 35% of the control when axotomy was prolonged more than 3 months, Concurrently, MU force and in increased exponentially, with a mean increase of threefold when axotomy was more than 3 months, which largely compensated for the reduction in the number of axons that rei nnervated the muscle. Consequently, muscles reinnervated by tibial mot or axons that had been axotomized up to 12 months produced as much for ce as those reinnervated by freshly axotomized tibial motor axons. Mus cle weight, size, and muscle fiber size were similar to those after im mediate nerve suture, Although prolonged axotomy does not compromise t he number of muscle fibers innervated by each axon, it does reduce the capacity of motor axons to regenerate and thus is an important contri buting factor to the poor functional recovery in delayed nerve repair.