EFFECT OF BIOTIN ON AMMONIA INTOXICATION IN RATS AND MICE

The effects of biotin on ammonia concentration in blood and brain were evaluated in hyperammonemic rats and mice. Rats were injected with 5 mmol/kg BW of ammonium acetate, and mice were injected with 10 mmol/kg BW. Increases in blood ammonia levels in rats 15-30 min after ammonia loading were prevented by treatment with 0.2 ml/100 g BW of biotin or 0.04 ml/100 g BW of arginine-glutamate with statistical significance. Blood ammonia levels after ammonia loading were lower, although not s ignificantly, in the arginine glutamate-treated rats than in the bioti n-treated animals. In mice also, increases in blood and brain ammonia levels after ammonia loading were prevented by the administration of b iotin. The decrease in brain glutamate and aspartate after ammonia loa ding was lower and the brain glutamine level was higher in biotin-trea ted mice than in the controls. These findings indicate the protective effect of biotin against ammonia intoxication.