HYPOOSMOTIC SWELLING TEST FOR QUALITY-CONTROL OF SPERM PREPARED FOR ASSISTED REPRODUCTION

This study evaluates the clinical usefulness of one additional paramet er for assessment of human sperm cell function in vitro-the hypoosmoti c swelling test. The hypoosmotic swelling test evaluated the functiona l integrity of the sperm plasma membrane. The investigation included a comparison of the hypoosmotic swelling test in samples containing mot ile and immotile spermatozoa and their correlation with the intrauteri ne insemination outcome. Motile spermatozoa expressed better membrane characteristics, without any importance of the hypoosmotic conditions. Positive correlation exists between HOS results and the outcome of IU I. This test can be a useful addition to the standard battery of semen analyzing tests.