Bh. Ewert et al., ANTIMYELOPEROXIDASE ANTIBODIES INDUCE NEUTROPHIL ADHERENCE TO CULTURED HUMAN ENDOTHELIAL-CELLS, Renal failure, 17(2), 1995, pp. 125-133
Antimyeloperoxidase autoantibodies are found in the sera of same patie
nts with glomerulonephritis and systemic vasculitis. Previously, we de
monstrated that they were able to stimulate neutrophils to damage cult
ured human endothelial cells. We now report that antimyeloperoxidase a
ntibodies are able to stimulate neutrophrils to adhere to cultured hum
an endothelial cells. Immunoglobulin G purified from myeloperoxidase-a
ntineutrophil cytoplasmic autoantibody positive sera increased adheren
ce to 331 +/- 60% of unstimulated controls. In a similar manner, rabbi
t antimyeloperoxidase enhanced neutrophil adherence. Stimulating the e
ndothelial cells with 10 mu g/mL endotoxin enhanced antimyeloperoxidas
e stimulated adherence. In the presence of a CD18 blocking antibody (M
oAb 60.3), antimyeloperoxidase-stimulated adherence was significantly
decreased. These results add further understanding to the antimyeloper
oxidase-stimulated neutrophil-endothelial cell interaction and further
support the hypothesis that antimyeloperoxidase autoantibodies are of
pathogenic import in glomerulonephritis and vasculitis.