PROSPECTIVE COMPARISON OF DIRECT IMMUNOFLUORESCENCE AND CONVENTIONAL STAINING METHODS FOR DETECTION OF GIARDIA AND CRYPTOSPORIDIUM SPP IN HUMAN FECAL SPECIMENS

Authors
ALLES AJ WALDRON MA SIERRA LS MATTIA AR
Citation
Aj. Alles et al., PROSPECTIVE COMPARISON OF DIRECT IMMUNOFLUORESCENCE AND CONVENTIONAL STAINING METHODS FOR DETECTION OF GIARDIA AND CRYPTOSPORIDIUM SPP IN HUMAN FECAL SPECIMENS, Journal of clinical microbiology, 33(6), 1995, pp. 1632-1634
Citations number
15
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
6
Year of publication
1995
Pages
1632 - 1634
Database
ISI
SICI code
0095-1137(1995)33:6<1632:PCODIA>2.0.ZU;2-X
Abstract
In a prospective comparative study, 2,696 consecutive fresh stool spec imens over the course of 1 year were examined for Giardia lamblia and Cryptosporidium parvum by using a direct immunofluorescent-monoclonal antibody stain (for unspun specimens) and conventional staining method s (chlorazol black E for Giardia cysts and modified Kinyoun acid-fast for Cryptosporidium oocysts). The direct immunofluorescent-monoclonal antibody method resulted in a significantly increased detection rate f or both giardia (118 versus 79 specimens, 49.4%; P = 0.006) and crypto sporidia (39 versus 23 specimens, 69.6%; P = 0.055).