Bj. Bricker et Sm. Halling, ENHANCEMENT OF THE BRUCELLA AMOS PCR ASSAY FOR DIFFERENTIATION OF BRUCELLA-ABORTUS VACCINE STRAINS S19 AND RB51, Journal of clinical microbiology, 33(6), 1995, pp. 1640-1642
Because the brucellosis eradication program uses slaughter and quarant
ine as control measures, it would benefit from faster methods of bacte
rial identification. Distinguishing vaccine strains from strains that
cause infections among vaccinated herds in the field is essential. To
accomplish this, our PCR-based, species-specific assay (B. J. Bricker
and S. M. Halling, J. Clin. Microbiol. 32:2660-2666, 1994) was updated
to identify Brucella abortus vaccine strains S19 and RB51. Three new
oligonucleotide primers were added to the five-primer multiplex Brucel
la AMOS PCR assay. Identification is based on the number and sizes of
six products amplified by PCR.