RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS PROVIDES RAPID DIFFERENTIATION OF METHICILLIN-RESISTANT COAGULASE-NEGATIVE STAPHYLOCOCCUS BACTEREMIA ISOLATES IN PEDIATRIC HOSPITAL

Coagulase-negative staphylococci (CoNS) are now recognized as the most common cause of nosocomial bacteremia in pediatric patients. Randomly amplified polymorphic DNA analysis was used to study the relationship s among 12 isolates of CoNS obtained from eight patients with catheter -related bacteremia in two distinct wards of our hospital and 6 epidem iologically unrelated strains. With this method, we were able to discr iminate isolates that otherwise mere indistinguishable by conventional criteria such as biochemical typing and antibiotic susceptibility pat terns. Our results indicated that there were episodes of cross-infecti ons among four patients in one ward but independent infectious episode s among four patients in the other ward. Randomly amplified polymorphi c DNA analysis is a rapid method which seems particularly well suited to the epidemiological study of CoNS isolates.