IN-VITRO EFFECT OF DEXTRAN BENZENE-TETRA-CARBOXYLATE HEMOGLOBIN ON HUMAN BLOOD RHEOLOGICAL PROPERTIES

While conducting pharmacological investigations into oxygen carriers, it is important to study the in vitro and in vivo theological behavior of blood cells in the presence of such preparations. With regard to t he original nature of human hemoglobin bound to benzene tetracarboxyla te substituted dextran (Dex-BTC-Hb), it seemed necessary to study its theological effect in a simulated in vitro hemorrhagic shock compensat ed by a blood substitute. The viscosity of substitutes was determined as well. as several theological parameters after 0, 3 and 6 hours incu bation periods of red blood cells with substitutes: viscosity of blood -substitute mixtures at different levels of plasma substitution erythr ocyte aggregation of blood-substitute mixtures by determining the velo city of rouleau formation and the cohesion of rouleau network. This wo rk yielded several observations: The viscosity of Dex-BTC-Hb was sligh tly higher than those of solutions of native Hb, Dex-BTC T10, Dextran 40 (Plasmacair(R)), modified fluid gelatin (Plasmion(R)) and hydroxyet hyl starch 200 (Elohes(R)). The substitution of a blood volume with De x-BTC-Hb, corresponding to a compensated 45% hemorrhagic shock, slight ly increased the viscosity of hemodiluted blood as compared to other s ubstitutes. In the presence of Dex-BTC-Hb, the aggregation of erythroc ytes appears to be increased as compared to standard solutions. Yet, t he effect was close to that of Plasmion(R) or Elohes(R)