EFFECT AND SAFETY OF LIPOSOME-ENCAPSULATED HEMOGLOBIN NEO RED-CELLS (NRCS) AS A PERFUSATE FOR TOTAL CARDIOPULMONARY BYPASS

We developed a liposome encapsulated hemoglobin named Neo Red Cells(NR C), NRC(1) readily circulates, (2) shows a high oxygen transport effic iency, and (3) has a strong capsule membrane. In this study, we evalua ted the effect of NRC as a priming solution for total cardiopulmonary bypass(TCPB) using a dog. The TCPB was started after removal of 57.1-7 3.3% of autologous blood and continued for 7 hours. During TCPB using NRC, the vascular resistance (VR) decreased to 1/4 of VR when red bloo d cells (RBC) were used. This change suggests that NRC, the viscosity of which is lower than that of RBC, reduced the load on the circulatio n system. The oxygen volume delivered by NRC was higher than that deli vered by RBC, resulting in a greater oxygen consumption with NRC. Duri ng TCPB using NRC, the serum LDH level was lower than that using RBC. So we concluded that NRC compensated for the reduction in the oxygen t ransport ability, which is a disadvantage of dilution TCPB, and furthe r increased the circulation improving effect and antihemolytic effect, which are advantages of the procedure. It, thus, enhanced both the sa fety and effectiveness of dilution TCPB.