EXPRESSION OF IL-2 AND IL-4 IN T-LYMPHOCYTES TRANSFORMED BY HERPESVIRUS SAIMIRI

Herpesvirus saimiri (H. saimiri) is a highly oncogenic lymphotropic he rpesvirus which can immortalize T lymphocytes and cause tumors in rabb its and New World monkeys. T cells infected with strain 484-77 of grou p C express four viral U-like small RNAs (HSUR1-4) and a 1.2-kb mRNA w hich encodes open reading frames ORF-1 and ORF-2. ORF-I encodes a coll agen-like oncoprotein. Deletion mutation analysis showed that ORF-1 an d ORF-2 are essential for IL-2 independent growth of human T cells inf ected with H. saimiri. An earlier study also demonstrated that H. saim iri-immortalized cells carry functional IL-2 receptors. The work prese nted in this report investigated whether IL-2 and IL-4 is produced by H. saimiri-immortalized T lymphocytes. Both IL-2 mRNA and IL-4 mRNA we re detected in various monkey T cells as well as human peripheral bloo d lymphocytes infected with wild-type H. saimiri. Secretion of IL-2 wa s suggested by cyclosporin A inhibition. IL-4 secretion by monkey T ce ll cultures was demonstrated by a bioassay and inhibition of bioactivi ty by an antibody to IL-4. The data also show that recombinant IL-4 st imulate H. saimiri-immortalized T cells; thus, IL-4 receptors are expr essed. However, antibodies to human IL-4, IL-4 receptor, or soluble IL -4 receptor did not curtail growth of transformed cells. T cells infec ted with ORF-1 and ORF-2 deletion mutants expressed no detectable IL-2 mRNA. ORF-1, ORF-2, HSUR1, and HSUR2, were all essential for expressi on of IL-4 mRNA. These data are consistent with the hypothesis that H. saimiri-immortalized monkey and human T lymphocytes proliferate throu gh autocrine secretion of IL-2 and that ORF-1, ORF-2, and HSUR sequenc es of the virus are involved in expression of lymphokines. (C) 1995 Ac ademic Press, Inc.