IDENTIFICATION OF A LOCUS ON MOUSE CHROMOSOME-17 ASSOCIATED WITH HIGH-AFFINITY CHOLINE UPTAKE USING BXD RECOMBINANT INBRED MICE AND QUANTITATIVE TRAIT LOCI ANALYSIS

Using the quantitative trait loci (QTL) approach, preliminary identifi cation has been made of a region on mouse chromosome 17 that influence s high-affinity choline uptake (HACU) in the mouse brain, The rate of HACU was measured in synaptosomes prepared from the frontal cortex, hi ppocampus, and striatum of C57BL/6J (B6), DBA/2J (D2), and 25 BXD reco mbinant inbred (RI) strains of mice, using a final concentration of 0. 5 mu M [H-3]choline, The strain means of HACU in each area were then c orrelated with the strain distribution pattern of each of 1300 known g enetic markers using a point biserial correlation and 0 (B6 allele) an d 1 (D2 allele), Correlations of P < 0.00001 were found between striat al HACU and chromosome 17 markers D17Tu50 and Tcp1. Correlations of P < 0.0001 were found between striatal HACU and chromosome 17 markers D1 7Leh66e, D17Leh119, D17Rp17e, Plg, D17Leh66d, Ckb-rs2, and Trp53-ps, Q TL analyses of HACU in the frontal cortex and hippocampus also reveale d correlations with these markers at the level of P < 0.05 and P < 0.0 1. These data suggest that at least one locus located on mouse chromos ome 17 near or between 6 and 13 cM from the centromere influences HACU in the striatum and possibly the frontal cortex and hippocampus of th e mouse. (C) 1995 Academic Press, Inc.