OPTIMIZATION OF PCR-BASED DIAGNOSTICS FOR HUMAN CYTOMEGALOVIRUS

Authors
VINOGRADSKAYA GR GORYSHIN IY BERLIN YA LANZOV VA
Citation
Gr. Vinogradskaya et al., OPTIMIZATION OF PCR-BASED DIAGNOSTICS FOR HUMAN CYTOMEGALOVIRUS, Journal of virological methods, 53(1), 1995, pp. 103-112
Citations number
25
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
53
Issue
1
Year of publication
1995
Pages
103 - 112
Database
ISI
SICI code
0166-0934(1995)53:1<103:OOPDFH>2.0.ZU;2-B
Abstract
Various techniques of DNA template preparation for the PCR-based analy sis of human CMV in biological fluids have been compared. Structural p olymorphism of a CMV DNA segment (part of the major immediate early ge ne) in clinical isolates is described; the molecular markers (nucleoti de substitutions, deletions, insertions) localized in the analyzed amp licon appear to be suitable for molecular-epidemiological studies. A s cheme of spreading of the molecular markers in the population is sugge sted.