DETERMINATION OF APRINDINE IN HUMAN PLASMA USING REVERSED-PHASE HPLC

Authors
MISZTAL G PRZYBOROWSKI L
Citation
G. Misztal et L. Przyborowski, DETERMINATION OF APRINDINE IN HUMAN PLASMA USING REVERSED-PHASE HPLC, Die Pharmazie, 50(3), 1995, pp. 187-188
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
Die Pharmazie → ACNP
ISSN journal
00317144
Volume
50
Issue
3
Year of publication
1995
Pages
187 - 188
Database
ISI
SICI code
0031-7144(1995)50:3<187:DOAIHP>2.0.ZU;2-B
Abstract
A rapid, simple, accurate method is presented for the determination of aprindine in human plasma. Liquid-liquid extraction of aprindine was carried out using diethyl ether. Amiodarone was applied as an internal standard. The samples were chromatographed on LiChrosorb RP-18 (10 mu m) column and the mobile phase was methanol/acetonitrile/phosphate bu ffer pH 2.5 (80:15:5). The detection was carried at 254 nm. The method was tested for linearity (range from 0.5 to 2.5 mu g/ml), recovery (c a. 90%) and precision (CV = 3.7%).