STUDY ON THE ACTIVITY AND KINETIC CHARACTERISTICS OF GLUCOAMYLASE IN W O MICROEMULSION SYSTEMS/

The activity and kinetic characteristics of glucoamylase has been inve stigated in W/O microemulsion systems of AOT/isooctane/buffer, CTAB/is ooctane/1-pentanol/buffer and TX-100/isooctane/1-pentanol/buffer, and compared with that in aquous solution. The effect of various parameter s, such as pH optimum, To ptimum and water content, on the activity of the enzyme in microemulsion was determined. The results obtained show that the structure of the microemulsion has strong effect on enzyme a ctivity compared to the k(cat) in aquous solution, the apparent turnov er mumber k'(cat) values were decreased in AOT, TX-100 based system, s ad in creased in CTAB based system.