ANNULAR APERTURE 2-PHOTON EXCITATION MICROSCOPY

Citation
Sw. Hell et al., ANNULAR APERTURE 2-PHOTON EXCITATION MICROSCOPY, Optics communications, 117(1-2), 1995, pp. 20-24
Citations number
16
Categorie Soggetti
Optics
Journal title
ISSN journal
00304018
Volume
117
Issue
1-2
Year of publication
1995
Pages
20 - 24
Database
ISI
SICI code
0030-4018(1995)117:1-2<20:AA2EM>2.0.ZU;2-T
Abstract
We investigate the effect of annular apertures in two-photon excitatio n confocal and non-confocal microscopy. In a theoretical study, we sho w that the use of annular apertures in two-photon excitation microscop y leads to resolution increase without diffraction fringes. We determi ne experimentally the axial resolution of annular aperture confocal an d non-confocal two-photon excitation microscopes and compare it with t hat of the standard two-photon excitation contrasts. Images of fluores cence beads obtained with a transmission annular aperture two-photon e xcitation microscope demonstrate the applicability of annular aperture s for enlarging the focal depth in high resolution two-photon excitati on imaging.