This study assessed intraobserver and interobserver variability in the
fluorescein dye disappearance test (DDT) and compared the DDT with a
modified Jones I test in a masked clinical trial in asymptomatic patie
nts. Seventy-six patients at the Jerry L. Pettis Memorial Veterans Adm
inistration Hospital, (152 eyes) who were without epiphora, high tear
lake, ectropion, or punctal stenosis in either eye underwent modified
Jones testing and the DDT. The latter was photographed at 5 minutes, a
nd the photos were assessed independently by three experts. Afterward,
these experts assessed all the data as a group and made a final asses
sment regarding patency. The final assessment of the panel was that 14
2 systems were patent, two were equivocal, and eight were obstructed.
The DDT interpretations of two physicians were in agreement with the f
inal assessment in 95% to 98% of eyes. The third physician had a syste
matic error in his interpretation of the DDT, causing his results to b
e only 73% accurate. Interobserver variability in the DDT was signific
ant at P < 0.00005. The Jones test was in agreement with the final ass
essment in 144 of 150 eyes, or 96%. When performed properly, the DDT a
nd the Jones I tests gave excellent results in normal patients.