H. Tao et al., C-NATRIURETIC PEPTIDE BUT NOT ATRIAL-NATRIURETIC-PEPTIDE INCREASES CYCLIC-GMP IN CEREBRAL ARTERIAL SMOOTH-MUSCLE CELLS, Life sciences, 56(26), 1995, pp. 2357-2365
Citations number
32
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
A-type natriuretic peptide (ANP) is found primarily in the heart and i
s released into the circulation. C-type (CNP) is found principally in
the brain and has also been detected in the systemic circulation. When
injected, both peptides produce vasodilatation most likely by elevati
on of guanosine 3'5'- cyclic monophosphate (cGMP) in smooth muscle cel
ls via two distinct receptors, NPR-A and NPR-B. In this present study,
we determined the effects of these two peptides on intracellular cGMP
in smooth muscle cells cultured from pig cerebral and peripheral arte
ries. In smooth muscle cells cultured from the left anterior descendin
g coronary artery, ANP and CNP increased cGMP with equal potency and e
fficacy (EC(50) for ANP and CNP, 3.6 +/- 0.2 x 10(-8) M and 6.7 +/- 0.
8 x 10(-8) M, respectively). In contrast, in smooth muscle cells from
cerebral arteries, ANP was without effect while CNP increased cGMP in
a concentration dependent manner (EC(50): 9.6 +/- 1.7 x 10(-8) M). Sti
mulation of the soluble guanylyl cyclase with either nitroglycerin or
nitroprusside was equivalent in the two cell types. The pattern of res
ponse of intracellular cGMP to CNP and ANP in isolated intact arteries
from brain and heart was similar to that found in the cultured cells.
These results suggest that smooth muscle cells in cerebral arteries e
xpress only NPR-B while cells from peripheral arteries can express bot
h NPR-A and NPR-B.