TROPHIC EFFECTS OF SUBSTANCE-P AND BETA-AMYLOID PEPTIDE ON DIBUTYRYL CYCLIC AMP-DIFFERENTIATED HUMAN LEUKEMIC (HL-60) CELLS

The neuropeptide substance P (SP) is a mediator of neurogenic inflamma tion. Also, beta-amyloid protein (beta AP) can directly activate the c ell types involved in inflammatory processes. The relationship between and SP and beta AP on their biological actions has attracted much int erest. SP is trophic in neuronal cells and protected them against the death induced by beta AP. In this study, we examined the effects of SP and beta-amyloid peptide on cell viability in neutrophil-like HL-60 c ells, by means of the WST-1 tetrazolium and lactate dehydrogenase (LDH ) release assays. The results showed that SP promoted the cell surviva l on neutrophil-like HL-60 cells in serum-free conditions. Also, beta- amyloid peptide showed trophic effects rather than toxic in these cell s in WST-1 assay, though it is reportedly toxic in neuronal cells.