M. Fontenayroupie et al., INCREASED PROLIFERATION OF BONE-MARROW-DERIVED FIBROBLASTS IN PRIMITIVE HYPERTROPHIC OSTEOARTHROPATHY WITH SEVERE MYELOFIBROSIS, Blood, 85(11), 1995, pp. 3229-3238
Pachydermoperiostosis or primary hypertrophic osteoarthropathy (HOA) i
s a rare congenital growth disorder of connective tissue, We report a
case of severe myelofibrosis in a patient with HOA. When cultured in v
itro, patient bone marrow-derived fibroblasts displayed a high prolife
rative potential with a shortened doubling time (24 hours v 36 to 48 h
ours for normal fibroblasts). The role of platelet-derived growth fact
or (PDGF), previously implicated in the pathogenesis of secondary acqu
ired myelofibrosis, was studied, HOA fibroblasts expressed an increase
d number of PDGF-BB binding sites (300,000 sites/cell v 200,000 sites/
cell for normal fibroblasts) without any modification of affinity. The
increased expression of PDGF-R beta appeared to result from an accele
rated rate of PDGF-R beta resynthesis with normal kinetics of endocyto
sis, As a consequence, a several-fold increase of PDGF-R beta tyrosine
kinase activity was observed, No autocrine mechanism of growth was su
spected as neither spontaneous PDGF-R beta autophosphorylation nor mit
ogenic activity in HOA fibroblast-conditioned medium was detected. Pat
ient serum and platelet lysate were less potent than controls in induc
ing [H-3]thymidine incorporation into HOA fibroblasts. This was incons
istent with a paracrine mechanism of growth, In vitro, human serum or
PDGF-BB were not more mitogenic for HOA than normal fibroblasts. High
levels of cyclin D1, a putative oncogene, were detected in serum-depri
ved HOA fibroblasts, Cyclin D1 overexpression could be implicated in t
he accelerated growth of these cells. Our results suggest that the mec
hanism of fibroblastic proliferation observed in this case of myelofib
rosis might differ from those reported in other acquired myeloprolifer
ative syndromes and could be associated with an intrinsic abnormality
of HOA fibroblast growth. (C) 1995 by The American Society of Hematolo
gy.