PERCENTAGE OF PHILADELPHIA-CHROMOSOME (PH)-NEGATIVE AND PH-POSITIVE CELLS FOUND AFTER AUTOLOGOUS TRANSPLANTATION FOR CHRONIC MYELOGENOUS LEUKEMIA DEPENDS ON PERCENTAGE OF DIPLOID-CELLS INDUCED BY CONVENTIONAL-DOSE CHEMOTHERAPY BEFORE COLLECTION OF AUTOLOGOUS CELLS
M. Talpaz et al., PERCENTAGE OF PHILADELPHIA-CHROMOSOME (PH)-NEGATIVE AND PH-POSITIVE CELLS FOUND AFTER AUTOLOGOUS TRANSPLANTATION FOR CHRONIC MYELOGENOUS LEUKEMIA DEPENDS ON PERCENTAGE OF DIPLOID-CELLS INDUCED BY CONVENTIONAL-DOSE CHEMOTHERAPY BEFORE COLLECTION OF AUTOLOGOUS CELLS, Blood, 85(11), 1995, pp. 3257-3263
We collected peripheral blood mononuclear cells and bone marrow cells
soon after recovery from conventional-dose chemotherapy-induced myelos
uppression and transplanted these cells into advanced chronic myelogen
ous leukemia (CML) patients after treating these patients with 120 mg/
kg cyclophosphamide, 750 mg/m(2) VP-16, and 1,020 cGy of total body ir
radiation (TBl). Of the 10 late chronic-phase patients and the eight a
ccelerated-phase CML patients evaluable posttransplant, 90% and 87%, r
espectively, remain alive posttransplant, whereas none of the three bl
ast crisis CML patients given this therapy remain alive posttransplant
. We measured the percentage of Philadelphia chromosome (Ph)-negative
cells in the autologous cells collected after conventional-dose chemot
herapy-induced myelosuppression before autologous transplant and in th
e marrow of these same CML patients after autologous transplantation o
f these cells into recipients treated with the cyclophosphamide, VP-16
, and TBl. A direct correlation (correlation coefficient = 0.91) was o
bserved between the level of Ph(+) cells in the transplanted cells and
the percentage of Ph(+) marrow cells after transplant in 21 patients
so transplanted. The data show that the chance of generating cytogenet
ic remissions posttransplant depends on the percentage of diploid cell
s in the preparations of autologous cells used for transplant and the
stage of disease of the patients at the time of collection of the auto
logous cells. (C) 1995 by The American Society of Hematology.