ENANTIOMER SEPARATION OF DNP-AMINO ACIDS BY CAPILLARY ELECTROPHORESISUSING CHIRAL BUFFER ADDITIVES

The direct enantiomeric analysis of six DNP-amino acids by capillary e lectrophoresis is reported. This study describes a comparison of sever al chiral buffer additives. Both the natural proteins BSA, AGP, ovomuc oid and casein, and the cyclic oligosaccharides alpha-, beta-, and gam ma-cyclodextrin were used as chiral selectors. For the first time, cas ein from goat milk was successfully applied for chiral discrimination. The influence of the modifier n-propanol on chiral discrimination is demonstrated with BSA as a buffer additive.