CALBINDIN(28KDA) AND CALMODULIN ARE HYPERABUNDANT IN RAT DENTAL ENAMEL CELLS - IDENTIFICATION OF THE PROTEIN PHOSPHATASE CALCINEURIN AS A PRINCIPAL CALMODULIN TARGET AND OF A SECRETION-RELATED ROLE FOR CALBINDIN(28KDA)

Enamel cells are likely to experience heavy demands for intracellular calcium homeostasis during the secretion and hypermineralization of de ntal enamel. Here, the two major high-affinity calcium-binding protein s in rat enamel epithelium were identified as calbindin(28 kDa) and ca lmodulin, using a microscale approach. Both proteins were hyperabundan t, totalling up to 2% of the soluble protein and surpassing the amount s in cerebellum the benchmark tissue. Calbindin(28 kDa) and calmodulin accounted for 26% of the total calcium-binding capacity in enamel cel l cytosol, under near physiological conditions. Numerous calmodulin-bi nding proteins were detected with an overlay assay, indicating that ca lmodulin has multiple major targets in enamel cells. The calcium/calmo dulin-regulated protein phosphatase, calcineurin, was identified as a principal calmodulin target constituting 0.1% of the soluble protein. Calmodulin and calcineurin were expressed constitutively, implying con tinued heavy usage of calcium/calmodulin-based and phosphorylation-bas ed signalling events throughout enamel cell development. Calbindin(28 kDa), in contrast, was expressed at fourfold higher levels in secretio n-phase cells than during the calcium-intensive hypermineralization ph ase, unexpectedly pointing to an important role associated with secret ion. Supporting this notion, immunoblots revealed that 33% of total (S DS-soluble) calbindin(28 kDa), was in the particulate fraction and pre dominantly associated with the Triton-insoluble cytoskeleton. Solubili sation of cytoskeletal calbindin(28 kDa) required high concentrations of NaCl or urea, indicating the existence of a high-affinity target li gand. The unusual abundance of calmodulin, calbindin(28 kDa) and calci neurin demonstrated here provides the first molecular evidence that en amel cells possess a strong capability for intracellular calcium homeo stasis. Since none of these proteins was up-regulated during enamel hy permineralization, it appears that other calcium-binding proteins are primarily involved in the putative transcellular passage of calcium.