COMBINED USE OF TEMPERATURE AND SOLVENT STRENGTH IN REVERSED-PHASE GRADIENT ELUTION .1. PREDICTING SEPARATION AS A FUNCTION OF TEMPERATURE AND GRADIENT CONDITIONS
Pl. Zhu et al., COMBINED USE OF TEMPERATURE AND SOLVENT STRENGTH IN REVERSED-PHASE GRADIENT ELUTION .1. PREDICTING SEPARATION AS A FUNCTION OF TEMPERATURE AND GRADIENT CONDITIONS, Journal of chromatography, 756(1-2), 1996, pp. 21-39
Citations number
39
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
It has been shown previously that computer simulation based on two ini
tial experiments can predict separation in reversed-phase gradient elu
tion as a function of gradient conditions (gradient steepness, gradien
t range and gradient shape) and column conditions (column length, flow
-rate and particle size). The present study extends this capability fo
r changes in temperature. Four initial experiments (two different grad
ient times, two different temperatures) provide input data that allow
predictions of separation as a function of temperature as well as grad
ient and column conditions. A semi-empirical relationship, t(R) = a+bT
, is able to relate gradient retention time t(R) to column temperature
T (other conditions constant). The accuracy of this approach has been
evaluated for 102 solutes and a variety of experimental conditions, i
ncluding the use of five different HPLC instruments (four different mo
dels).