COMBINED USE OF TEMPERATURE AND SOLVENT STRENGTH IN REVERSED-PHASE GRADIENT ELUTION .1. PREDICTING SEPARATION AS A FUNCTION OF TEMPERATURE AND GRADIENT CONDITIONS

Citation
Pl. Zhu et al., COMBINED USE OF TEMPERATURE AND SOLVENT STRENGTH IN REVERSED-PHASE GRADIENT ELUTION .1. PREDICTING SEPARATION AS A FUNCTION OF TEMPERATURE AND GRADIENT CONDITIONS, Journal of chromatography, 756(1-2), 1996, pp. 21-39
Citations number
39
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
756
Issue
1-2
Year of publication
1996
Pages
21 - 39
Database
ISI
SICI code
Abstract
It has been shown previously that computer simulation based on two ini tial experiments can predict separation in reversed-phase gradient elu tion as a function of gradient conditions (gradient steepness, gradien t range and gradient shape) and column conditions (column length, flow -rate and particle size). The present study extends this capability fo r changes in temperature. Four initial experiments (two different grad ient times, two different temperatures) provide input data that allow predictions of separation as a function of temperature as well as grad ient and column conditions. A semi-empirical relationship, t(R) = a+bT , is able to relate gradient retention time t(R) to column temperature T (other conditions constant). The accuracy of this approach has been evaluated for 102 solutes and a variety of experimental conditions, i ncluding the use of five different HPLC instruments (four different mo dels).