COMBINED USE OF TEMPERATURE AND SOLVENT STRENGTH IN REVERSED-PHASE GRADIENT ELUTION .3. SELECTIVITY FOR IONIZABLE SAMPLES AS A FUNCTION OF SAMPLE TYPE AND PH

Authors
ZHU PL DOLAN JW SNYDER LR HILL DW VANHEUKELEM L WAEGHE TJ
Citation
Pl. Zhu et al., COMBINED USE OF TEMPERATURE AND SOLVENT STRENGTH IN REVERSED-PHASE GRADIENT ELUTION .3. SELECTIVITY FOR IONIZABLE SAMPLES AS A FUNCTION OF SAMPLE TYPE AND PH, Journal of chromatography, 756(1-2), 1996, pp. 51-62
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
756
Issue
1-2
Year of publication
1996
Pages
51 - 62
Database
ISI
SICI code
Abstract
The ability of temperature and gradient steepness to change band spaci ng has been investigated for several ionizable samples that include 8 substituted benzoic acids, 9 substituted anilines, 22 basic drugs, 9 s tructurally-related herbicide impurities, 7 chlorophylls and 72 peptid es and proteins. Mobile phase pH was also varied to determine the effe ct of sample ionization on temperature and gradient-steepness selectiv ity.