E. Simoalfonso et al., PERFORMANCE OF A SERIES OF NOVEL N-SUBSTITUTED ACRYLAMIDES IN CAPILLARY ELECTROPHORESIS OF DNA FRAGMENTS, Journal of chromatography, 756(1-2), 1996, pp. 255-261
Citations number
30
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
DNA separations by capillary electrophoresis in viscous solutions of n
ovel polymers, made with Omega-hydroxyl, N-substituted acrylamides (no
tably N-acryloyl amino propanol, AAP and N-acryloyl amino butanol, AAB
) are evaluated. Whereas in standard poly(acrylamide), at 6% concentra
tion, the theoretical plate number (N) does not exceed 500 000, in 6%
poly(AAP) N reaches 922 000 and in 6% poly(AAB) N values as high as 1
200 000 are obtained. Also, copolymers of AAP and AAB give N values in
excess of 1 million plates. The two novel monomers (AAP and AAB) rema
in extremely stable during alkaline hydrolysis and display very good h
ydrophilicity, while being devoid of the noxious habit of autopolymeri
zation and auto-reticulation exhibited by the previous monomer of this
series (N-acryloyl amino ethoxy ethanol). The reasons for such a good
performance of the Omega-substituted acrylamide derivatives could be
that their polymers may form hydrogen bonds via their distal -OH group
during DNA separation.