CLONING, SEQUENCING AND EXPRESSION OF THE L5, L21, L27A, L28, S5, S9,S10 AND S29 HUMAN RIBOSOMAL-PROTEIN MESSENGER-RNAS

During systematic analysis of the mRNAs expressed in a human colorecta l carcinoma with the aim of evidencing new makers of the disease (Frig erio et al. (1995), in press), we isolated several clones correspondin g to homologs of rat ribosomal protein mRNAs L5, L21, L27a, L28, S5, S 9, S10 and S29. Because expression of several mRNAs encoding ribosomal proteins was found strongly altered during colorectal carcinogenesis, sequence of these transcripts, not previously described in human, was completed and their expression analyzed. Northern blot analysis of RN As extracted from colorectal cancer and adjacent normal tissue from 6 patients revealed in all of them perturbations of expression in cancer , compared to normal. No correlation could however be made between the level of expression and the severity of the disease. Yet, abnormal pa tterns with additional larger transcripts were observed in some patien ts for rpL5, rpL28 and rpS10.