COVERAGE OF CHROMOSOME-6 BY CHROMOSOME MICRODISSECTION - GENERATION OF 14 SUBREGION-SPECIFIC PROBES

Human chromosome 6 has been subdivided by chromosome microdissection i nto 14 unique regions. Following microdissection, polymerase chain rea ction (PCR) amplification of dissected DNA was performed using a unive rsal primer to generate subregion-specific probes that provided comple te coverage of chromosome 6. All 16 microdissections have been regiona lly assigned along chromosome 6 by fluorescence in situ hybridization (FISH) using biotin-labeled dissected DNA hybridized to G-banded norma l metaphase chromosomes. These probes can be used as region-specific p aints to generate unique ''bar codes'' and for analysis of chromosome alterations involving chromosome 6 that are unidentifiable by conventi onal banding analysis.