SIMULTANEOUS RAW STARCH HYDROLYSIS AND ETHANOL FERMENTATION BY GLUCOAMYLASE FROM RHIZOCTONIA-SOLANI AND SACCHAROMYCES-CEREVISIAE

Crude glucoamylase preparation from Rhizoctonia solani was used to sac charify raw and cooked starch. Various concentrations of potato starch and wheat flour from 10-40%, w/v were used for mashing but 30% was fo und to be the optimal and economical. The saccharified mash yielded 5. 89%, v/v ethanol in a simultaneous saccharification and fermentation p rocess using a yeast strain Saccharomyces cerevisae (SC-39) at 35 degr ees C for 4 days. Removal of inhibitory substances from the fermenting broth through dialysis caused considerable increase in ethanol produc tion.