NEUTRAL LIPIDS OF FUNGUS ZOOPHTHORA - LABORATORY CULTURE MEDIA AND NEUTRAL LIPIDS OF THE FUNGUS ZOOPHTHORA (ERYNIA) NEOAPHIDIS

Cultivation of Zoophthora (Erynia) neoaphidis, iii comparison with oth er species of Zoophthora genus, requires more complex nitrogen source. The best mycelium growth was obtained when 8 day old mycelium was use d as an inoculum for liquid culture. On the basis of Gas Chromatograph y/Mass Spectrometry (GC/MS) methods, the structures of the neutral lip ids produced by this fungus species, were determined. Decanoic acid wa s the most characteristic component (over 45 %). The level of hexadeca noic and octadecanoic acid was relatively lower, although their contri bution as ''bonded'' acid in diacylglyce-ride (DG) and triacylglycerid e (TG) fractions, was significant. Contrary to Zoophthora (Erynia) ovi spora, the presence of campesterol and/or dihydrobrassicosterol was pr oved.