THE COMPARISON OF ANALYSIS OF NITROGEN-COMPOUNDS USING HPLC AND CLASSICAL COLUMN CHROMATOGRAPHY

Normal-phase HPLC has been applied in semi-preparative scale in order to separate nitrocompounds and azaarenes fraction from the airborne pa rticulate matter extract This procedure has been compared with methodo logy comprising classical column chromatography and nitroarenes analys is as their amide derivatives. It has been found that HPLC fractions s eparated directly from the extract are very complex and their analysis by GC-MS technique is more complicated than the analysis of nitro- an d azaarenes concentrates obtained by classical column chromatography. Fewer separate nitrogen compounds were identified in HPLC fractions th an in concentrates of these compounds separated by the other method. I n the extract of dusts collected in the region of Olkusz the following compounds were identified: nitrofluorene, nitroarenes of M.W.=247, az afluorene, acridine, quinoline, benzoquinolines, azapyrenes, benzacrid ines. The concentrations of nitrogen compounds determined in HPLC frac tions and nitro- and azaarenes concentrates are close to each other.