INTERINDIVIDUAL DIFFERENCES IN THE CONCENTRATION OF 1-HYDROXYPYRENE-GLUCURONIDE IN URINE AND POLYCYCLIC AROMATIC HYDROCARBON-DNA ADDUCTS INPERIPHERAL WHITE BLOOD-CELLS AFTER CHARBROILED BEEF CONSUMPTION

Biological markers of internal dose and macromolecular dose from PAHs provide a potential means of assessing environmental exposure to PAHs through inhalation, ingestion and percutaneous absorption, In this stu dy we examined the time course and interindividual variation of 1-hydr oxypyrene-glucuronide (1-OHP-gluc) excretion in urine and PAH-DNA addu ct formation in peripheral white blood cells (WBCs) after charbroiled (CB) beef consumption, As a marker of internal dose, 1-OHP-gluc was me asured in human urine using immunoaffinity chromatography and synchron ous fluorescence spectroscopy, PAH-DNA adducts were measured in WBCs b y enzyme-linked immunosorbent assay (ELISA) in order to assess macromo lecular dose, Ten healthy non-smoking males consumed identical amounts of CB beef on five consecutive days, Multiple blood and urine samples were collected before, during, and after the feeding period, The morn ing after the first day of CB beef consumption, individual urinary con centrations of 1-OHP-gluc increased 10- to 80-fold (range: 2.0-16.6 pm ol/ml urine) above pre-feed baseline concentrations (0.23 +/- 0.11 pmo l/ml) in the 10 subjects, 1-OHP-gluc concentration decreased to near b aseline levels by 24-72 h after CB beef consumption ended, In contrast , PAH-DNA adducts in WBCs increased markedly in only four of 10 subjec ts during or after CB beef consumption, Significant interindividual va riation was observed for both urinary 1-OHP-gluc concentration (P < 0. 001 by Kruskal-Wallis) and PAH-DNA adduct levels (P < 0.005) during th e feeding period, The mean urinary 1-OHP-gluc concentration for each s ubject during and immediately after (days 2-8) the feeding period was significantly correlated with their mean PAH-DNA adduct level in WBCs during the same time period (Spearman r = 0.79, P < 0.01), Evidence of segregation of the subjects into separate response groups based on le vel of urinary 1-OHP-gluc was observed, suggesting that discrete deter minants may regulate the absorption, metabolism and/or excretion of in gested pyrene.