S-PHASE ARREST AND INDUCTION OF MULTINUCLEATED CELLS AFTER EXPOSURE TO ULTRAVIOLET-RADIATION

Citation
H. Banrud et al., S-PHASE ARREST AND INDUCTION OF MULTINUCLEATED CELLS AFTER EXPOSURE TO ULTRAVIOLET-RADIATION, Carcinogenesis, 16(5), 1995, pp. 1087-1094
Citations number
59
Categorie Soggetti
Oncology
Journal title
ISSN journal
01433334
Volume
16
Issue
5
Year of publication
1995
Pages
1087 - 1094
Database
ISI
SICI code
0143-3334(1995)16:5<1087:SAAIOM>2.0.ZU;2-T
Abstract
The effects of UVA (365 nm) and UVB (297/302 nm) radiation on cellular proliferation, cell cycle progression, aneuploidy and multinucleus in duction have been studied in two different fibroblast cell lines; V79 Chinese hamster lung fibroblasts and 3T3 Swiss albino mouse fibroblast s. UVA and UVB were found to inhibit proliferation of the cells in a f luence-dependent manner, This inhibition was due to a temporary accumu lation of cells in the S phase of the cell cycle, as determined by flo w cytometry of UV-irradiated V79 cells, The UVA- and UVB-induced S pha se delay was observed a few hours after irradiation and by 48 h post-i rradiation the cells had recovered from cell cycle arrest. For WA, but not for UVB, the elongation of S phase was followed by a small accumu lation of cells in the G(2) phase, After exposure to UVA and a post-ir radiation time long enough for the cells to recover from cell cycle ar rest, a large proportion of the cells were polyploid, with two or more nuclei, Multinucleated cells were not, however, induced by UVB irradi ation.