H. Banrud et al., S-PHASE ARREST AND INDUCTION OF MULTINUCLEATED CELLS AFTER EXPOSURE TO ULTRAVIOLET-RADIATION, Carcinogenesis, 16(5), 1995, pp. 1087-1094
The effects of UVA (365 nm) and UVB (297/302 nm) radiation on cellular
proliferation, cell cycle progression, aneuploidy and multinucleus in
duction have been studied in two different fibroblast cell lines; V79
Chinese hamster lung fibroblasts and 3T3 Swiss albino mouse fibroblast
s. UVA and UVB were found to inhibit proliferation of the cells in a f
luence-dependent manner, This inhibition was due to a temporary accumu
lation of cells in the S phase of the cell cycle, as determined by flo
w cytometry of UV-irradiated V79 cells, The UVA- and UVB-induced S pha
se delay was observed a few hours after irradiation and by 48 h post-i
rradiation the cells had recovered from cell cycle arrest. For WA, but
not for UVB, the elongation of S phase was followed by a small accumu
lation of cells in the G(2) phase, After exposure to UVA and a post-ir
radiation time long enough for the cells to recover from cell cycle ar
rest, a large proportion of the cells were polyploid, with two or more
nuclei, Multinucleated cells were not, however, induced by UVB irradi
ation.