LARGE-CELL ANAPLASTIC (LCA) CD30 KI-1 POS ITIVE STLV-1 ASSOCIATED T-TYPE MALIGNANT-LYMPHOMAS (BABOON MALIGNANT-LYMPHOMA MODEL)/

Large-cell anaplastic malignant lymphoma of T-type (T-LCAL) as an orig inal form among human non-Hodgkin's malignant lymphomas in Updated Kie l Classification (1988) was defined. In human pathology T-LCAL present s a considerable diagnostic difficulties and is not associated with HT LV except some cutaneous forms. Comparative examination was carried ou t in 5 cases of baboon T-LCAL as a part of long-term investigation of the lymphoma outbreak in Sukhumi monkey stock. In tumor-bearing baboon s routine morphological methods, immuno-morphological phenotyping of l ymphoma cells with panel of MoAT and polyclonal antibodies to T- and B -lymphoid cell antigens and antigens of cell activation, including CD3 0 antigen, in cell suspension and on cryostate sections were used. STL V-provirus integration in DNA of lymphoma cells in PCR was determined. Diagnostically important morphological features of baboon and human T -LCAL include pronounced anaplasia and anisocytosis of CD30-positive l arge blast cells, tumour cell tendency to infiltrate sinuses and vascu lar walls. The presence of T-cell markers (CD2, CD3, CD4, CD8 in our c ases) and antigens of cell activation (CD25, HLA-DH) were found. In DN A of baboon lymph node lymphomas (also in the cases without skin invol vement) env- and tax-fragments of STLV-1 provirus were detected.