ELECTROPHORETIC TRANSFER OF PROTEIN PIGMENT COMPLEXES FROM NONDENATURING GELS TO ELECTRON-MICROSCOPY GRIDS

Different fractions of a mixture of protein-pigment complexes have bee n separated from one another by non-denaturing gel electrophoresis. Th ese complexes were prepared for observation by inserting electron micr oscope grids directly into the focused bands of the pigment-protein co mplexes and resuming electrophoresis for a brief time, so that the com plexes were deposited onto the grids. It was found that complexes depo sited from each band exhibited distinctly different appearances. It wa s also found that the exact conditions of electrophoretic deposition o nto the grids affected the appearance of the complexes. The protein-pi gment complexes were characterized additionally by spectroscopy and de naturing gel electrophoresis.