POSTISCHEMIC CHANGES IN CARDIAC SARCOPLASMIC-RETICULUM CA2- A POSSIBLE MECHANISM OF ISCHEMIC PRECONDITIONING( CHANNELS )

We investigated the modifications of cardiac ryanodine receptors/sarco plasmic reticulum Ca2+ release channels occurring in ischemic precondi tioning. In an isolated rat heart model, the injury produced by 30 min utes of global ischemia was reduced by preexposure to three 3-minute p eriods of global ischemia (preconditioning ischemia). The protection w as still present 120 minutes after preconditioning ischemia but disapp eared after 240 minutes. Three 1-minute periods of global ischemia did not provide any protection. In the crude homogenate obtained from ven tricular myocardium, the density of [H-3]ryanodine binding sites avera ged 372+/-18 fmol/mg of protein in the control condition, decreased 5 minutes after preconditioning ischemia (290+/-15 fmol/mg, P<.01), was still significantly reduced after 120 minutes (298+/-17 fmol/mg,, P<.0 5), and recovered after 240 minutes (341+/-21 fmol/mg). Three 1-minute periods of ischemia did not produce any change in ryanodine binding. The K-d for ryanodine (1.5+/-0.3 nmol/L) was unchanged in all cases. I n parallel experiments, the crude homogenate or a microsomal fraction was passively loaded with Ca-45, and Ca2+-induced Ca2+ release was stu died by the quick filtration technique. In both preparations, the rate constant of Ca2+-induced Ca2+ release decreased 5 and 120 minutes aft er preconditioning ischemia (homogenate values: 19.7+/-1.4 and 18.9+/- 0.9 s(-1) vs a control value of 25.4+/-1.7 s(-1) P<.05 in both cases) and recovered after 240 minutes (23.0+/-1.9 s(-1)). The Ca2+ dependenc e of Ca2+-induced Ca2+ release was not affected by preconditioning isc hemia. In conclusion, changes in sarcoplasmic reticulum Ca2+-release c hannels occur after brief ischemia and reperfusion, are closely correl ated with the development of myocardial protection Versus sustained is chemia, and might play a role in the pathogenesis of ischemic precondi tioning.