ESTABLISHMENT OF AN EXPERIMENTAL-INFECTION PROTOCOL FOR THE FLAT OYSTER, OSTREA-EDULIS, WITH THE INTRAHAEMOCYTIC PROTOZOAN PARASITE, BONAMIA-OSTREAE - APPLICATION IN THE SELECTION OF PARASITE-RESISTANT OYSTERS
D. Hervio et al., ESTABLISHMENT OF AN EXPERIMENTAL-INFECTION PROTOCOL FOR THE FLAT OYSTER, OSTREA-EDULIS, WITH THE INTRAHAEMOCYTIC PROTOZOAN PARASITE, BONAMIA-OSTREAE - APPLICATION IN THE SELECTION OF PARASITE-RESISTANT OYSTERS, Aquaculture, 132(3-4), 1995, pp. 183-194
The availability of purified Bonamia ostreae suspensions and the devel
opment of an inoculation protocol enabled experimental infections with
the European flat oyster, Ostrea edulis, to be carried out. Different
infectious doses were tested and the kinetics of bonamiasis studied,
The 50% infectious dose (ID), determined from inoculations with 10 to
10(6) B. ostreae per individual, was estimated to be 80 000 parasites
for 3-year-old oysters, This 50% ID was used to screen for parasite-re
sistant oysters, Preliminary results suggested that the F-1 progeny of
oysters that survived previous outbreaks of bonamiasis were more resi
tant to B. ostreae.