USE OF CHEMICALLY-MODIFIED SILICA WITH BETA-DIKETOAMINE GROUPS FOR SEPARATION OF ALPHA-LACTOALBUMIN FROM BOVINE-MILK WHEY BY AFFINITY-CHROMATOGRAPHY

Silica gel surface was chemically modified with beta-diketoamine group s by reacting the silanol from the silica surface with 3-aminopropyl-t riethoxysilane and 3-bromopentanedione, With this material, copper ion s were adsorbed from aqueous solutions, The chemical analysis of the s ilica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g (-1) of organic groups attached to the support and 0.63 mmol g(-1) of copper, This material was used as a stationary phase in IMAC (immobili zed metal affinity chromatography), to separate alpha-lactoalbumin fro m bovine milk whey, The results showed an efficient separation in the chromatographic column, The possibility of reutilization of the statio nary phase was also investigated. (C) 1997 Academic Press