GADOLINIUM BLOCKS MECHANOELECTRIC TRANSDUCER CURRENT IN CHICK COCHLEAR HAIR-CELLS

Authors
KIMITSUKI T NAKAGAWA T HISASHI K KOMUNE S KOMIYAMA S
Citation
T. Kimitsuki et al., GADOLINIUM BLOCKS MECHANOELECTRIC TRANSDUCER CURRENT IN CHICK COCHLEAR HAIR-CELLS, Hearing research, 101(1-2), 1996, pp. 75-80
Citations number
25
Categorie Soggetti
Neurosciences,Acoustics
Journal title
Hearing research → ACNP
ISSN journal
03785955
Volume
101
Issue
1-2
Year of publication
1996
Pages
75 - 80
Database
ISI
SICI code
0378-5955(1996)101:1-2<75:GBMTCI>2.0.ZU;2-S
Abstract
We investigated the effects of gadolinium ion (Gd3+) On th, mechano-el ectrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependen t manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coeffic ient of 1.14 and a dissociation constant (K-D) of 1.01 x 10(-5) M. Ada ptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.