GENETIC DIFFERENTIATION OF CANDIDA-ALBICANS STRAINS BY MIXED-LINKER POLYMERASE CHAIN-REACTION

Restriction fragment length polymorphisms (RFLPs) of C. albicans were visualized using a mixed-linker polymerase chain reaction (PCR) and a C. albicans-specific primer (C. albicans 1059-bp species-specific repe at sequence). The method produced 10-14 bands of between 200 and 1400 bp on agarose gel electrophoresis and ethidium bromide staining. It ga ve comparable results to Southern blot hybridization with good reprodu cibility and the time required for the production of typing profiles w as reduced to less than 2 days.