Ja. Perezpons et al., INDUCTION AND PRELIMINARY CHARACTERIZATION OF INTRACELLULAR BETA-GLUCOSIDASES FROM A CELLULOLYTIC STREPTOMYCES STRAIN, FEMS microbiology letters, 128(3), 1995, pp. 235-239
The cellulolytic actinomycete Streptomyces sp. QM-B814 posesses an int
racellular beta-glucosidase system which is induced by cellobiose and
carboxymethylcellulose. Maximal beta-glucosidase activity was attained
8-10 h after inducer addition to exponential phase growing cultures.
The induction is depressed in the presence of glucose. The system is c
omposed of two electrophoretically different beta-glucosidase forms sh
owing relative molecular masses of about 60 and 35 kDa, and pI values
in the range 4.2-4.5. Both beta-glucosidases are synthesized de novo.
The enzymes share substrate preference and are both inhibited by delta
-gluconolactone and p-chloromercuribenzoate. The induction pattern and
glucose inhibition are similar for both enzymes.