MEASUREMENT OF SERUM-LIPID PEROXIDATION DURING EXERCISE USING 3 DIFFERENT METHODS - DIENE CONJUGATION, THIOBARBITURIC ACID REACTIVE MATERIAL AND FLUORESCENT CHROMOLIPIDS

Serum lipid peroxidation was measured by three different methods (dien e conjugation, DC; thiobarbituric acid reactive material, TBARM; and f luorescent chromolipids, FC) during physical exercise of different dur ation. The aim of the study was to investigate which of the three meth ods is the most representative in measuring oxidative stress after phy siological stimulus (physical exercise) in man. In general, diene conj ugation is a measure of early events of lipid peroxidation reactions, TBARM measures end products of lipid peroxidation and FC are products formed by reaction of end products of lipid peroxidation with the amin o groups. In trial I, 8 subjects ran 1 km with maximal speed; in trial II, 7 subjects ran 10 km with maximal speed; in trial III, 9 subjects ran 27 km with maximal speed, and in trial IV, 5 subjects cycled 8 da ys in competition, altogether 1061 km biking. in each trial venous blo od samples were taken before and immediately after the exercise. In tr ials II, III, and IV serum DC concentration increased by 14% (P < 0.05 ), 11% (P < 0.01), and 25% (P < 0.05), respectively, during the exerci se. FC or TBARM did not change in the trials. We conclude that DC as a measure of the early phase of lipid peroxidation may be less affected by protective antioxidant functions than FC and TBARM methods, which both measure end products of lipid peroxidation. Therefore, DC may be the most sensitive of the three methods to estimate serum lipid peroxi dation induced by exercise in man. We also suggest that the apparently conflicting results of serum lipid peroxidation in earlier studies ma y be caused by use of different methods to measure lipid peroxidation.